TECHNICAL DATA SHEET 741
Page 5 of 6
Microring
®
GV
Cat. #24655
(50 rings/pkg)
Microring
®
GV is a filter paper ring used for identification of Gardnerella vaginalis. The ring comes complete with 4 individual printed
tips containing various antimicrobial reagents. Each tip of the ring contains one of the following reagents; MTZ (metronidazole), TRM
(trimethoprim), STZ (sulphathiazole), and BIL, (bile salt). Each tip performs as a single identification disc because of an isolating
hydrophobic barrier. On blood agar, Gardnerella is identified by sensitivity to 3 designated tips and resistance to 1 tip.
Procedure
1. Spread sample of test organism onto a plate containing Columbia Agar with blood.
2. Using sterile forceps, carefully place Microring
®
GV in the center of the plate. Press each tip gently to ensure contact with surface.
3. Incubate plate for 48 hours at 37° C in humid atmosphere containing 5% CO2.
4. Following incubation, growth patterns around each tip are read and used to identify the organism.
Species
Gardnerella Vaginalis
Vaginal Lactobacilli
Cat -ve Corynebacter
Mtz
S
R
R
Trm
S
R
R
Stz
R
V
V
Bil
S
R
R
Bile 5mg
Metronidazole
50 mcg
MTZ = metronidazole, TRM = trimethoprim, STZ = sulphathiazole, BIL = bile salt
S = Susceptible, R = Resistant, V = Variable
Storage & Handling
Store at 4° C, avoid prolonged exposure to direct sunlight.
Microring
®
GV should be handled with forceps.
Avoid contact with skin to prevent contamination.
Rings maintain a shelf life 12 of months from manufacture date.
Sulphathiazole
1mg
Trimethoprim
5 mcg
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
© Polysciences, Inc.
Active: Jan/2007
Data Sheet #741
1
GEC [ GENERAL ELECTRIC COMPANY ]
