TECHNICAL DATA SHEET 741
Microring
®
XV Interpretation
Species
H. influenzae
H. aegypticus
H. parainfluenzae
H. haemolyticus
H. parahaemolyticus
Growth factor requirements
X
V
+
+
+
–
+
–
+
+
+
+
Synthesis of
porphyrins from ala
–
–
+
–
+
Haenikysis on
horse blood
–
–
–
+
+
Page 2 of 6
X = Haemin, V = NAD and
δ-aminolaevulinic
acid (ALA)
Storage & Handling
Store Microring
®
XV at 2-8°C
Rings maintain a shelf life of 24 months from manufacture date.
Microring
®
AN
Cat. #24645
(50 rings/pkg)
Microring
®
AN is a filter paper ring used for the identification of Non-sporing Anaerobes. It has 6 tips which are impregnated with a
range of antibiotics for the identification of species of anaerobic Gram-negative bacilli and can distinguish Gram-positive cocci from
Gram-negative cocci. Gram-negative anaerobic rods are found in more than half of clinical specimens containing anaerobes. Although
difficult to perform precise identifications, in practice most groups can be presumptively identified from their antibiotic susceptibility
profile, which is the basis of the Microring
®
AN test scheme.
Procedure
1. Spread sample of test organism onto a plate containing anaerobic agar.
2. Using sterile forceps, carefully place Microring
®
AN in the center of the plate.
Press each tip gently to ensure contact with surface.
3. Incubate for 24-48 hours at 35-37° C under anaerobic conditions.
4. Following incubation, growth patterns around each tip are read and used
to identify the organism. Zone diameters of 15mm or greater are considerd
susceptible, while those less than 15mm are regarded as resistant.
Code
E
RP
CO
PG
K
VA
Color
Red
Dark red
White
Pink
Salmon
Blue
Antibiotic
Erythromycin
Rifampicin
Colistin
Penicillin G
Kanamycin
Vancomycin
Amount
60ug
15ug
10ug
2 units
1000ug
5ug
Identification Table
Species
B. Fragilis
Prevotella
melaninogenicus
Prevotella oralis
B. ureolyticus
E RP CO PG K VA
S
S
S
S
S
S
S
S
R
V
S
S
R
S
S
S
R
R
R
S
R
R
R
R
Aesculin
hydrolysis
+
–
+
–
Growth
in bile
+
–
–
–
Indole
+
–
–
–
Oxidase positive.
Black pigmented colonies with brick
red fluorescence.
Other details
E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin
S = Sensitive, R = Resistant
Microring
®
AN
continues - pg 3
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
© Polysciences, Inc.
Active: Jan/2007
Data Sheet #741
1
GEC [ GENERAL ELECTRIC COMPANY ]
