CONNECTION EXAMPLES
GSimultaneous Measurement with a PMT Module and an Imaging Camera
The following is an example of simultaneous measurement in which the two A10921 objective lens mounts are attached to the
A10858 stage block. The lower (inverted) part excites the sample using laser light and measures the fluorescence of the sample
using the PMT module. The upper (upright) part illuminates the observation area using a xenon arc lamp and uses an imaging
camera to observe the observation area and the fluorescence signal.
A plano-convex lens and a fluorescence filter are installed in front of the PMT module to isolate the fluorescence. The plano-convex
lens converges the beam of light. If this causes a problem in the fluorescence filter's wavelength shift, place the filter in front of the
plano-convex lens.
So that there is no interference with the PMT module's measurement, use a wavelength for the imaging camera's illumination that is
different from both the excitation wavelength and the fluorescence wavelength. For example, if you use a 488 nm laser, use a DM505
dichroic mirror for the PMT module and a 530/40 bandpass filter for the emission filter. On the imaging camera side, use a 600 nm
longpass filter for illumination and put a laser-blocking filter in front of the camera.
LIGHT GUIDE A10014-35-0110
XENON LAMP
LIGHT GUIDE ADAPTER*
L9588-03
(COMMERCIALLY AVAILABLE)
C-MOUNT LIGHT GUIDE MOUNT*
(COMMERCIALLY AVAILABLE)
600 nm LONGPASS FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01
TUBE LENS BLOCK A10859-01
STAGE BLOCK A10858
OBJECTIVE
LENS MOUNT
A10921
EM-CCD
or
ICCD CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
LASER-BLOCKING FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
C-MOUNT CUBE BEAMSPLITTER* (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR)
C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
C-MOUNT RING A9865
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
OBJECTIVE
LENS MOUNT
A10921
PMT MODULE
FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213-01
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
(FOR THE CONNECTOR)
PLANO-CONVEX LENS (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
BEAM ALIGNER BLOCK A10760
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
OPTICAL FIBER (FC CONNECTOR)
LASER
* Example: Edmund Optics
•C-Thread Light Guide Mounting Adapter
•Fiber Optic Light Guide Adapter
•C-Mounted Cube Beamsplitter
NOTE: The A10858 stage block comes with one A10921 objective lens mount.
To perform simultaneous measurement as shown above, you will need to purchase an additional A10921.
TPMOC0222EA
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HAMAMATSU [ HAMAMATSU CORPORATION ]
